Environmental Genomics Facility
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EGF
Equipment
The Environmental Genomics Facility provides access to the following equipment:
The Environment Genomics Facility provides access to the following equipment:
Quick Links
Description
The Aligent TapeStation 4150 is a compact, automated electrophoresis system designed for DNA and RNA sample quality control. Utilizing ScreenTape technology, this system offers a simplified workflow for nucleic acid analysis. It also has the capacity to determine the RNA Integrity Number (RIN) of RNA samples, as well as the concentrations of both DNA and RNA.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 226
Additional Information:
Techniques
- Quantification and quality of DNA and RNA samples
- RIN values for RNA
Software
- TapeStation Controller
- TapeStation Analysis
Resources
Description
The Ion GeneStudio S5 System is a semiconductor-based sequencing platform used for Next-Generation Sequencing (NGS). The system allows for high-throughput projects of up to 600bp length amplicons. Paired with the Ion Chef for chip loading, the Ion GeneStudio S5 System provides users with a simplified workflow for obtaining high-quality sequencing data.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 226
Additional Information:
Techniques
- Ion-based Next-Generation Sequencing
Software
- Torrent Suite Software
Resources
Real-Time PCR System
Description
The Applied Biosystems QuantStudio 12K Flex Real-Time PCR System is a flexible, high-throughput quantitative PCR (qPCR) platform that allows samples to be analyzed using multiple fluorescent dyes. The system is outfitted with interchangeable block formats including 96-well, 384-well and OpenArray plate. Combined with the AccuFill System for automated sample loading, the QuantStudio 12K Flex Real-Time PCR System is ideal for researchers needing scalable and streamlined throughput research.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 226
Additional Information:
Techniques
- Fluorescent quantitative PCR
Software
Resources
Description
The Tecan Spark is a multimode microplate reader equipped with fluorescence, absorbance and luminescence modes for sample testing. This robust system is designed for a wide range of applications, from running rapid quantitative and qualitative tests of DNA/RNA/cDNA to conducting ELISAs.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 224
Additional Information:
Techniques
- ELISAs
- DNA/RNA/cDNA concentration and quality checks
Software
Resources
Description
The eDNA room is a contaminant free space equipped with a Biological Safety Cabinet (BSC), anteroom for decontamination prior to entering, and all materials and equipment required to extract DNA/RNA and prepare first round PCRs/qPCRs.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 128
Additional Information:
Techniques
- Environmental DNA/RNA extraction
- Preparation for first round PCRs/qPCRs
Description
A room dedicated to running gel electrophoresis and completing gel extractions. An Azure 200 imaging system and transilluminator are available to image gels run on the Avantor gel rigs.
Location
Great Lakes Institute for Environmental Research (GLIER)
Room # 132A
Additional Information:
Techniques
- Gel imaging of PCR products
Software
- Azure 200