Environmental Genomics Facility
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EGF
Services / User Fees
The Environmental Genomics Facility offers the following services for a fee:
Note. User fees are charged to help offset some of the costs associated with operating and maintaining the core facility. The funds generated by these fees are key to allowing the network of core facilities within NUCLEUS to effectively support research.
DNA Extraction
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| Bead-based DNA extraction | $2-5/sample | $3-10/sample | $4-12/sample |
Service Notes
- The cost of DNA extraction will vary based on method and whether it needs to be completed in the eDNA room. Please contact the Environmental Genomics Facility for more details.
RNA Extraction
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| RNA extraction | $275/24 samples | $400/24 samples | $450/24 samples |
Service Notes
- The cost is for a QIAGEN kit-based RNA extraction and includes quality/quantification on the Tecan Spark Multimode Microplate Reader.
NGS Preparation
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| NGS preparation (Full) | $1,000/plate | $1,400/plate | $1,680/plate |
| NGS preparation (1st round PCRs submitted) | $800/plate | $1,120/plate | $1,300/plate |
Service Notes
- The cost of NGS preparation is per 96-well plate.
- Full NGS preparation service includes target PCR, bead-cleaning, barcoding, pooling, and QA/QC of final library. NGS preparation can also begin at bead cleaning if first round PCRs are submitted.
NGS Run
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| NGS run (400bp or less) | $2,600/chip | $3,500/chip | $4,200/chip |
| NGS run (600bp or less) | $2,700/chip | $3,800/chip | $4,600/chip |
Service Notes
- NGS run (400bp or less) – The cost is for an entire chip, which can house anywhere from 384-768 samples at a time. Multiple libraries/projects can be combined on a single chip to decrease each user’s cost.
- NGS run (600bp or less) – The cost is for an entire chip, which can house up to 384 samples. Multiple libraries/projects can be combined on a single chip to decrease each user’s cost.
- All NGS runs are conducted on the Ion Torrent systems.
NGS Bioinformatic Analysis
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| NGS bioinformatic analysis | $1,000/chip | $1,200/chip | $1,680/chip |
Service Notes
- The cost for NGS bioinformatic analysis includes all steps needed to analyze results from the Ion Torrent S5 system per sequencing chip. This process involves utilizing bioinformatic pipelines to demultiplex and BLAST against the NCBI (or custom) database.
qPCR – TaqMan OpenArray Technology
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| qPCR utilizing Thermo Fisher custom-designed OpenArray assays | $160/chip | $225/chip | $275/chip |
Service Notes
- The cost includes the running of the chip; it does not include the cost of ordering the chips. The chips are ordered on a project-by-project basis, and the cost is fully covered by the user. The cost of the chips themselves will vary depending on market cost at the time of ordering. Please contact the Core Manager of the Environmental Genomics Facility for more information.
qPCR – SYBR Green or TaqMan Primer&Probe
| Service | Internal Academic Rate (UWindsor) | External Academic Rate (non-UWindsor) | Industry Rate |
|---|---|---|---|
| qPCR utilizing SYBR Green or custom TaqMan Primer/Probe assay | Contact for quote | Contact for quote | Contact for quote |
Service Notes
- The cost will depend on the number of samples, chemistry selected, and market cost for reagents at the time of project. Please contact the Core Manager of the Environmental Genomics Facility for more information.